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一株酵母菌釀造柿子酒的發(fā)酵特性及其序列鑒定
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    摘要:

    通過三因素二次回歸旋轉(zhuǎn)組合試驗(yàn)設(shè)計(jì),研究了發(fā)酵溫度、接種量、發(fā)酵液初始pH值對(duì)菌株P(guān)S2釀造柿子酒發(fā)酵特性的影響,得出了柿子酒質(zhì)量與工藝因素間的回歸模型。結(jié)果表明:3個(gè)因素對(duì)柿子酒質(zhì)量均有極顯著影響(p<0.01=,最佳工藝參數(shù)是發(fā)酵溫度為22℃,接種量為10.4%,pH值為3.4。利用ITS1-5.8S-ITS2序列分析鑒定該菌株的分類水平,結(jié)果表明菌株

    Abstract:

    PS2為Saccharomyces cerevisiae HA6(登錄號(hào)SC09327)。 The effects of fermentation temperature, inoculum volume and pH on persimmon wine quality were investigated by three-factor second order regression rotation composite design. The result showed that the regression equation obtained between the factors and persimmon wine quality had a high reliability. All the three factors had significant influence on persimmon wine quality (p<0.01=. The optimum parameters were 22℃ of fermentation temperature, 10.4% of inoculum volume and 3.4 of pH, respectively. Then, the identification of the strain PS2 was performed by ITS1-5.8S-ITS2 rDNA sequencing. The results showed that the ITS1-5.8S-ITS2 rDNA sequence homology was 98% and the genetic distance 0.001 2 between the strain PS2 and Saccharomyces cerevisiae HA6(SC09327), which indicated that they belonged to the same strain.

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彭幫柱,岳田利,龍明華,袁亞宏,趙志華.一株酵母菌釀造柿子酒的發(fā)酵特性及其序列鑒定[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2007,38(3):90-94.[J]. Transactions of the Chinese Society for Agricultural Machinery,2007,38(3):90-94.

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