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磁場(chǎng)和抑制劑對(duì)蓮藕多酚氧化酶反應(yīng)動(dòng)力學(xué)的影響
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    摘要:

    通過添加Vc和外加磁場(chǎng)處理，研究蓮藕多酚氧化酶（PPO）反應(yīng)動(dòng)力學(xué)的變化規(guī)律。以鄰苯二酚為底物，蓮藕PPO褐變反應(yīng)動(dòng)力學(xué)符合米氏方程所描述的單底物酶促反應(yīng)動(dòng)力學(xué)，Km為0.775mol/L，Vmax為11.150U/min。Vc對(duì)PPO有較強(qiáng)的抑制作用，反應(yīng)動(dòng)力學(xué)參數(shù)Km為0.081mol/L，Vmax為3.243U/min，呈現(xiàn)反競(jìng)爭(zhēng)性抑制，表現(xiàn)為褐變出現(xiàn)滯后，隨Vc濃度的增大滯后時(shí)間逐漸增長(zhǎng)，且呈現(xiàn)S型趨勢(shì)，并建立了相應(yīng)的反應(yīng)方程。不同磁場(chǎng)強(qiáng)度下，隨著處理時(shí)間的變化，PPO活性呈現(xiàn)波動(dòng)性。3.17A/m磁場(chǎng)強(qiáng)度下處理4h的酶液其反應(yīng)動(dòng)力學(xué)曲線表現(xiàn)為S型，并建立了相應(yīng)的反應(yīng)方程。

    Abstract:

    The change law of reaction dynamics was experimental studied through adding Vc to lotus root polyphenol-oxidase and dealing with lotus root polyphenol-oxidase (PPO) by magnetic field. The experimental results indicated that the reaction kinetic of enzymatic browning was accordant with Michaelis equation with catechol substrate, which Km and Vmax were 0.775 mol/L and 11.150U/min respectively. Vc had an inhibiting effect on lotus root PPO, which Km and Vmax were 0.081mol/L and 3.243U/min respectively. The browning was lagged and the lag time increased gradually with the rise in the concentration of the inhibitors, which was an anticompetitive inhibition. The PPO activity expressed a fluctuation at different magnetic field strengths and times. At 3.17A/m magnetic field strength for 4 hours, the reaction kinetics curve was a wave-type curve. The kinetic equations were established respectively.

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高夢(mèng)祥,胡翠翠,嚴(yán)奉偉,張長(zhǎng)峰.磁場(chǎng)和抑制劑對(duì)蓮藕多酚氧化酶反應(yīng)動(dòng)力學(xué)的影響[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2008,39(1):78-81.[J]. Transactions of the Chinese Society for Agricultural Machinery,2008,39(1):78-81.

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