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高壓脈沖電場(chǎng)提取樺褐孔菌多糖的試驗(yàn)
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    摘要:

    針對(duì)高壓脈沖電場(chǎng)（PEF）對(duì)樺褐孔菌多糖提取率的影響進(jìn)行了單因素和正交試驗(yàn)，并與熱堿法、微波輔助提取法、超聲波輔助提取法進(jìn)行了對(duì)比試驗(yàn)。結(jié)果表明：當(dāng)電場(chǎng)強(qiáng)度為30kV/cm、脈沖數(shù)為6、液料比為25mL/g、pH值為10時(shí)，樺褐孔菌多糖的提取率達(dá)到49.8%，是熱堿提取法的1.67倍，是微波輔助提取法的1.12倍，多糖的純度是超聲輔助提取法的1.40倍。應(yīng)用PEF技術(shù)提取樺褐孔菌中的多糖，提取時(shí)間短（12μs），提取率高，提取物中雜質(zhì)少，是一種有效的樺褐孔菌多糖提取方法。

    Abstract:

    Extraction of polysaccharide from Inonotus obliquus by way of applying high intensity pulsed electric fields (PEF) was studied. Through single factor experiments and orthogonal experiment, the effect of PEF on polysaccharide extraction rate (ER) was discussed, and the comparison of alkali extraction method (TAEM), microwave-assist extraction method (MAEM) and ultrasonic-assist extraction method (UAEM) has been done. A set of optimal parameters (PEF strength=30kV/cm, pulse number=6, pH=10, liquid/solid ratio=25mL/g) were tested and analyzed, at which the ER could be up to 49.8%, 1.67 times of that by using TAEM, 1.12 times of MAEM, and the purity of polysaccharide was 1.40 times of UAEM. The experiments proved that PEF technique is an effective way to extract polysaccharide from the Inonotus obliquus, with higher extraction rate, shorter time (12μs), and less impurities.

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殷涌光,崔彥如,王婷.高壓脈沖電場(chǎng)提取樺褐孔菌多糖的試驗(yàn)[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2008,39(2):89-92.[J]. Transactions of the Chinese Society for Agricultural Machinery,2008,39(2):89-92.

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