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不同來(lái)源酶快速檢測(cè)有機(jī)磷農(nóng)藥的靈敏度比較
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    摘要:

    以商品乙酰膽堿酯酶（來(lái)自家蠅）和自制的雞肝乙酰膽堿酯酶、鯉魚(yú)腦乙酰膽堿酯酶為研究對(duì)象，比較3種不同濃度的有機(jī)磷農(nóng)藥（氧樂(lè)果、敵百蟲(chóng)和敵敵畏）對(duì)這3種酶的酶活抑制率。結(jié)果表明，3種農(nóng)藥對(duì)家蠅乙酰膽堿酯酶的抑制率最高；對(duì)雞肝和鯉魚(yú)腦乙酰膽堿酯酶的酶活抑制率比家蠅低； 3種乙酰膽堿酯酶對(duì)氧樂(lè)果都不敏感。在檢測(cè)精度要求不高的情況下可用鯉魚(yú)腦酶作為敵敵畏的快速檢測(cè)用酶，用雞肝酶作為氧樂(lè)果的快速檢測(cè)用酶。家蠅AChE是快速檢測(cè)敵百蟲(chóng)的敏感酶。

    Abstract:

    The commercial acetylcholinesterase (AChE) from Musca domestica, and the acetylcholinesterase made by the researchers themselves from fowl liver and cyprinoids brain, were used for pesticides detection. The inhibition of AChE activity under different concentrations of omethoate, trichlorfon and dichlorvos were compared. The results show that the inhibition of AChE activity of Musca domestica is the highest of the three above. Three kinds of Acetylcholinesterase are not sensitive to omethoate. AChE from cyprinoids brain can be used for rapid detection of dichlorvos in the case of high detection precision is not needed. AChE from fowl liver can be used for rapid detection of trichlorfon.  

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王小瑜,孫霞,王相友,孫冬霞.不同來(lái)源酶快速檢測(cè)有機(jī)磷農(nóng)藥的靈敏度比較[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2008,39(9):83-86.[J]. Transactions of the Chinese Society for Agricultural Machinery,2008,39(9):83-86.

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