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This paper demonstrated the detecting of chlorpyrifos at trace level in pesticide solution based on near infrared (NIR) method. Forty seven samples were prepared by simulating vegetables and fruits ingredient, thirty seven of which were used for quantitative analysis. The range of chlorpyrifos consistency was from 0.005mg/kg to 0.100mg/kg, Other samples with no pesticide were used for cluster analysis. The results showed that NIR method is appropriate to analyze the mixing solution containing traces of chlorpyrifos (between 0.008mg/kg and 0.090mg/kg).