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The temperature profiles in spatial and temporal field inside the glasshouse are coupled to outside climates. Especially, the discrete ordinate (DO) radiation model was applied to solve the temperature profiles inside the glasshouse. The 3-D numerical simulation was done based on the CFD technique for the natural ventilated Venlo glasshouse, and the measurement was conducted to validate the CFD model. The results showed that the simulation values agreed well with test values about the average temperature in the empty glasshouse for the different time, the max relative error and the average error was 11.3% and 7.6%, respectively. The spacial temperature grades were obvious inside the glasshouse. A homogeneous temperature field was obtained at crop level, the average temperature was about 28.5～28.8℃. The DO radiation model was proved effective in modeling the effect of solar radiation on the thermal climates inside the glasshouse.