亚洲一区欧美在线,日韩欧美视频免费观看,色戒的三场床戏分别是在几段,欧美日韩国产在线人成

超聲處理對(duì)大豆親脂蛋白結(jié)構(gòu)及溶解性的影響
作者:
作者單位:

作者簡介:

通訊作者:

中圖分類號(hào):

基金項(xiàng)目:

中國博士后科學(xué)基金面上項(xiàng)目(2018M631902)


Effect of Ultrasonic Treatment on Structure and Solubility of Soybean Lipophilic Protein
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 圖/表
  • |
  • 訪問統(tǒng)計(jì)
  • |
  • 參考文獻(xiàn)
  • |
  • 相似文獻(xiàn)
  • |
  • 引證文獻(xiàn)
  • |
  • 資源附件
  • |
  • 文章評(píng)論
    摘要:

    為探明超聲處理對(duì)大豆親脂蛋白(Lipophilic protein, LP)結(jié)構(gòu)及溶解性的影響,將分離提取的LP在不同條件下進(jìn)行超聲處理,采用十二烷基硫酸鈉-聚丙烯酰胺凝膠電泳(SDS-PAGE)、傅里葉變換紅外光譜(FITR)、內(nèi)源和外源熒光強(qiáng)度、差示掃描量熱法(DSC)分析超聲處理對(duì)LP結(jié)構(gòu)及溶解性的影響。結(jié)果表明:SDS-PAGE顯示,超聲處理并不能改變LP的分子量分布,但能改變亞基的含量;紅外光譜及內(nèi)源、外源熒光光譜顯示,不同的超聲處理?xiàng)l件對(duì)LP的二、三級(jí)結(jié)構(gòu)均會(huì)產(chǎn)生不同程度的影響,隨著超聲強(qiáng)度的增加,α螺旋先減少、后增加,β折疊先增加、后減少,在360W超聲功率下處理10min時(shí)內(nèi)源、外源熒光強(qiáng)度最強(qiáng);在360W超聲功率下處理10min和240W超聲功率下處理20min時(shí)對(duì)LP的熱穩(wěn)定性、溶解性的改善效果最佳,變性溫度提高6~7℃,溶解度提高約20個(gè)百分點(diǎn),而超聲功率過大時(shí)則會(huì)對(duì)LP的功能性質(zhì)產(chǎn)生負(fù)面影響。通過試驗(yàn)得出最佳超聲條件為:在360W超聲功率下處理10min,LP理化性質(zhì)最適合應(yīng)用于食品工業(yè)。

    Abstract:

    Aiming to investigate the effects of ultrasonic treatment on the structure and solubility of soybean lipophilic protein (LP). The extracted LP was ultrasonically separated under different conditions, and the effect of ultrasonic treatment on the structure and solubility of LP was analyzed by using sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy (FITR), endogenous, exogenous fluorescence intensity and differential scanning calorimetry (DSC). The results showed that SDS-PAGE showed that the ultrasonic treatment could not change the molecular weight distribution of LP, but could change the content of subunits, infrared spectrum and endogenous and exogenous fluorescence spectra. It was showed that different ultrasonic treatment conditions had different effects on the second and third structures of LP. With the increase of ultrasonic intensity, αhelix was firstly decreased and then increased, βfold was increased first and then decreased. The endogenous and exogenous fluorescence intensity was the strongest when treated at 360W ultrasonic power for 10min, and the thermal stability and solubility of LP were improved after treatment with 360W ultrasonic power for 10min and 240W ultrasonic power for 20min. The denaturation temperature was increased by 6~7℃, the solubility was increased by 20 percentage points, and it would have a negative impact on the functional properties of LP when the ultrasonic power was too large. Combined with the test results, it was concluded that the physicochemical properties of LP were most suitable for food applications after treatment at 360W ultrasonic power for 10min.

    參考文獻(xiàn)
    相似文獻(xiàn)
    引證文獻(xiàn)
引用本文

李楊,王迪瓊,齊寶坤,鐘明明,徐清清,謝鳳英.超聲處理對(duì)大豆親脂蛋白結(jié)構(gòu)及溶解性的影響[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2020,51(2):357-362,373. LI Yang, WANG Diqiong, QI Baokun, ZHONG Mingming, XU Qingqing, XIE Fengying. Effect of Ultrasonic Treatment on Structure and Solubility of Soybean Lipophilic Protein[J]. Transactions of the Chinese Society for Agricultural Machinery,2020,51(2):357-362,373.

復(fù)制
分享
文章指標(biāo)
  • 點(diǎn)擊次數(shù):
  • 下載次數(shù):
  • HTML閱讀次數(shù):
  • 引用次數(shù):
歷史
  • 收稿日期:2019-05-15
  • 最后修改日期:
  • 錄用日期:
  • 在線發(fā)布日期: 2020-02-10
  • 出版日期:
文章二維碼