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龍眼多糖樹脂脫色工藝優(yōu)化
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Optimization of De-coloration Technology for Polysaccharides from Longan Pulp
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    以脫色率、多糖保留率、蛋白質(zhì)去除率及3個指標(biāo)的加權(quán)綜合評分比較8種樹脂對龍眼多糖的脫色效果，并篩選出效果較好的離子交換樹脂D301-F，通過單因素和正交試驗(yàn)進(jìn)一步優(yōu)化其脫色工藝條件。結(jié)果表明：當(dāng)龍眼多糖溶液質(zhì)量分?jǐn)?shù)為4％時，選用離子交換樹脂D301-F，在料液比為0.16g/mL、溶液pH值為5、溫度為50℃條件下的脫色率可達(dá)90.21%，多糖保留率為85.75%，蛋白質(zhì)去除率為73.12%。龍眼多糖中的色素可能主要以帶負(fù)電荷的非極性小分子為主，采用離子交換樹脂脫色是一種有效的純化方法。

    Abstract:

    De-coloration rate, polysaccharides holding rate and protein removal rate of eight resins on longan polysaccharides were studied. The results showed that anion exchange resin D301-F presented good de-colorizing effects. De-colorizing experiments were carried out by single experiment and orthogonal experiment. The data indicated that the colorants were most possibly small-molecular-weight substances with negative charge. The optimum technological parameters were as follows: the ratio for resin and crude polysaccharides solution (the mass ratio was 4%) was 0.16g/mL under the condition of 50℃ and pH value 5. The de-coloration rate was 90.21%, the polysaccharides holding rate was 85.75%, the protein removal rate was 73.12%. 

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易陽,張名位,廖森泰,魏振承,唐小俊,張瑞芬.龍眼多糖樹脂脫色工藝優(yōu)化[J].農(nóng)業(yè)機(jī)械學(xué)報,2010,41(8):146-150.Optimization of De-coloration Technology for Polysaccharides from Longan Pulp[J]. Transactions of the Chinese Society for Agricultural Machinery,2010,41(8):146-150.

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