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黃瓜葉片葉綠素含量近紅外光譜無損檢測
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國家高技術(shù)研究發(fā)展計(jì)劃(863計(jì)劃)資助項(xiàng)目(2008AA10Z208)、國家自然科學(xué)基金資助項(xiàng)目(60901079)和江蘇省普通高校研究生科研創(chuàng)新計(jì)劃資助項(xiàng)目


NIR Spectra in Non-invasive Measurement of Cucumber Leaf Chlorophylls Content
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    摘要:

    為了簡化黃瓜葉片葉綠素光譜模型和提高模型預(yù)測精度,采用聯(lián)合區(qū)間偏最小二乘法(SiPLS)結(jié)合凈分析物法(NAS)提取近紅外光譜的特征信息,建立了黃瓜葉片葉綠素光譜模型。收集了110片新鮮黃瓜葉片,用近紅外光譜儀采集光譜數(shù)據(jù)后立刻用化學(xué)分析方法測定葉綠素含量。原始光譜經(jīng)過SNV預(yù)處理和子區(qū)間總數(shù)優(yōu)化后,將全光譜均勻劃分為29個(gè)子區(qū)間,用聯(lián)合區(qū)間偏最小二乘法優(yōu)選出4個(gè)特征子區(qū)間,在上述特征子區(qū)間的基礎(chǔ)上,用凈分析物法分離光譜中同葉綠素相關(guān)的光譜信息,并結(jié)合線性回歸法建立了葉綠素光譜模型。模型對(duì)應(yīng)的校正集相關(guān)系數(shù)Rc、校正均方根誤差、預(yù)測集相關(guān)系數(shù)Rp和預(yù)測均方根誤差分別為0.9472、0.0795mg/g、0.9250和0.0906mg/g。結(jié)果表明:聯(lián)合區(qū)間偏最小二乘法結(jié)合凈分析物法能夠有效提取葉綠素的特征光譜信息,提高模型精度的同時(shí)降低其復(fù)雜度。

    Abstract:

    To improve and simplify the prediction model of chlorophylls content of cucumber leaves, synergy interval partial least squares (SiPLS) and net analyte signal (NAS) were combined to search for optimized informative spectral wavelengths about chlorophylls content from NIR spectra of cucumber leaves, then spectral model was developed on the basis of chlorophylls contents. One hundred and ten cucumber leaves were selected to collect NIR spectra and chlorophylls content according to chemical analysis. The spectra were preprocessed by SNV method and divided into 29 intervals, among which 4 subsets, i.e. No 3, 4, 5, 15 were selected by SiPLS. Then NAS was used to characterize the net signals of chlorophyll from cucumber leaf spectra which were used for regression variables of NAS model. The NAS calibration model was obtained with the correlation coefficient Rc of 0.9472, root mean square error of calibration of 0.0795mg/g , the prediction coefficient Rp of 0.9250 and root mean square error of prediction of 0.0906mg/g. It proves that SiPLSNAS could determine optimal variables in NIR spectra and improve the accuracy of model. 

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石吉勇,鄒小波,趙杰文,毛罕平,王開亮,陳正偉.黃瓜葉片葉綠素含量近紅外光譜無損檢測[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2011,42(5):178-182,141. Shi Jiyong, Zou Xiaobo, Zhao Jiewen, Mao Hanping, Wang Kailiang, Chen Zhengwei. NIR Spectra in Non-invasive Measurement of Cucumber Leaf Chlorophylls Content[J]. Transactions of the Chinese Society for Agricultural Machinery,2011,42(5):178-182,141.

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