野生酸棗果硒多糖純化與光譜分析

doi:10.3969/j.issn.1000-1298.[year].[issue].[sequence]

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野生酸棗果硒多糖純化與光譜分析
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                        10.3969/j.issn.1000-1298.[year].6.[sequence]
                    
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基金項目:中國科學院知識創(chuàng)新項目(KZCX2—XB2—05—01)和“十一五”國家科技支撐計劃資助項目（2008BAD98B08）

Purification and Corresponding Spectroscopic Analysis of Selenium Polysaccharide in Sour Jujube Fruits

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采用熱水浸提、乙醇沉淀法獲得酸棗果硒多糖粗品。苯酚—硫酸法測其多糖含量為（92.6±4.72）mg/g，原子熒光光度法測其硒含量為（17.8±2.05）μg/kg。DEAE—25纖維素和Sephadex G—75凝膠層析柱進一步分離純化得白色絮狀多糖。薄層層析和高效陰離子交換色譜法分析其單糖組成。結(jié)果表明：該多糖是由鼠李糖、阿拉伯糖、半乳糖、葡萄糖、木糖等單糖組成，其摩爾比為3.210∶8.36∶5.61∶0.96∶1.82。紅外光譜分析表明具有明顯的多糖特征吸收峰，核磁共振波譜表明該多糖結(jié)構(gòu)主要為β-糖苷鍵連接的吡喃型葡聚糖。

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The crude polysaccharide was extracted from sour jujube fruits using hot water extraction followed by ethanol precipitation.The content of crude polysaccharide was (92.6±4.72) mg/g by phenol-sulfuric acid assay, and selenium content of the polysaccharide was (17.8±2.05) μg/kg by fluorescence spectrophotometry method. After further purified by DEAE—52 and Sephadex G—75 column chromatography, a white and flocculent polysaccharide was obtained. After hydrolysis with 2M trifluoroacetic acid, TLC plates was detected and high performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was further analyzed. A comparative study of the two methods showed that the polysaccharide was composed of rhamnose, xylose, arabinose, glucose and galactose with a ratio of 3.21∶8.36∶5.63∶0.96∶1.82. The selenium polysaccharide exhibited typical absorption of polysaccharide characterized by Infrared spectroscopy (IR) spectroscopy;1H NMR and 3C NMR analysis indicated that the structure of selenium polysaccharide was pyranoid glucan withβ-glycosidic bond.

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孫延芳,梁宗鎖,單長卷,Viernstein H, Unger F.野生酸棗果硒多糖純化與光譜分析[J].農(nóng)業(yè)機械學報,2011,42(6):148-151,147. Sun Yanfang, Liang Zongsuo, Shan Changjuan, Viernstein H, Unger F. Purification and Corresponding Spectroscopic Analysis of Selenium Polysaccharide in Sour Jujube Fruits[J]. Transactions of the Chinese Society for Agricultural Machinery,2011,42(6):148-151,147.

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