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高酸值米糠油酶法酯化脫酸研究
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國家自然科學(xué)基金資助項(xiàng)目（30871739、31071556）;安徽省教育廳重點(diǎn)科研資助項(xiàng)目（KJ2007A099）;安徽省重點(diǎn)攻關(guān)項(xiàng)目（07010302117）


Deacidification of High-acid Rice Bran Oil by Enzymatic Esterification
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    摘要:

    對脂肪酶Novozym 435催化高酸值米糠油酯化脫酸進(jìn)行了研究。利用響應(yīng)面分析法(RSM)優(yōu)化脫酸條件。根據(jù)Box—Benhnken中心組合試驗(yàn)設(shè)計原理對試驗(yàn)條件進(jìn)行優(yōu)化，在分析各因素顯著性及其交互作用的基礎(chǔ)上，得出米糠油酯化脫酸最佳工藝參數(shù)為：酶質(zhì)量分?jǐn)?shù)1.1%、甘油添加量0.42mg、溫度56.4℃、反應(yīng)時間23.2h。實(shí)際測得脫酸后米糠油酸值（KOH）由56.mg/g降為5.04mg/g，游離脂肪酸酯化率達(dá)到91%，與模型預(yù)測值基本相符。

    Abstract:

    Deacidification of high-acid rice bran oil catalyzing by esterification using lipase Novozym 435 were studied. According to response surface method (RSM) and Box—Benhnken experimental design, the best conditions of enzymatic esterification were optimized based on the analysis of significant factors and their interactions. The optimum conditions were：enzyme concentration of 1.1%, initial glycerol of 0.42mg, reaction temperature of 56.4℃, the reaction time of 23.2h. Under the conditions，rice bran oil acid value（KOH） decreased from 56mg/g to 5.04mg/g，the esterification rate of free fatty acid（FFA） reached to 91% and matched the prediction value by model.

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吳聰,曾慶梅,靳靖,魏春燕,黃博英.高酸值米糠油酶法酯化脫酸研究[J].農(nóng)業(yè)機(jī)械學(xué)報,2011,42(6):156-160. Wu Cong, Zeng Qingmei, Jin Jing, Wei Chunyan, Huang Boying. Deacidification of High-acid Rice Bran Oil by Enzymatic Esterification[J]. Transactions of the Chinese Society for Agricultural Machinery,2011,42(6):156-160.

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