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基于近紅外高光譜圖像的黃瓜葉片色素含量快速檢測(cè)
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國(guó)家自然科學(xué)基金資助項(xiàng)目(60901079);國(guó)家高技術(shù)研究發(fā)展計(jì)劃(863計(jì)劃)資助項(xiàng)目(2008AA10Z208)


Rapid Detection of Cucumber Leaves Pigments Based on Near Infrared Hyper-spectral Image Technology
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    摘要:

    利用高光譜圖像技術(shù)和高效液相色譜法(HPLC)快速檢測(cè)了新鮮黃瓜葉中葉綠素a、葉綠素b、β-胡蘿卜素和葉黃素4種色素含量。采集了120片黃瓜葉的近紅外高光譜圖像數(shù)據(jù)以及用HPLC精確測(cè)定黃瓜葉中色素含量;提取高光譜圖像中50×50像素感興趣區(qū)域(ROI)的平均光譜與4種色素含量分別建立偏最小二乘(PLS)預(yù)測(cè)模型;為了提高模型的穩(wěn)定性和預(yù)測(cè)精度,分別采用區(qū)間偏最小二乘(iPLS)、向后區(qū)間偏最小二乘(BiPLS)和聯(lián)合區(qū)間偏最小二乘(SiPLS)對(duì)各種色素對(duì)應(yīng)的特征波段進(jìn)行優(yōu)選,同時(shí)對(duì)光譜劃分?jǐn)?shù)進(jìn)行了優(yōu)化。結(jié)果表明BiPLS和SiPLS對(duì)應(yīng)模型的預(yù)測(cè)效果較好,對(duì)葉綠素a、葉綠素b、β-胡蘿卜素和葉黃素4種色素的預(yù)測(cè)集相關(guān)系數(shù)RP分別為0.8257、0.8134、0.8116、0.8262。

    Abstract:

    Rapid detection the content of chlorophyll a, chlorophyll b, β-carotene and lutein on fresh cucumber leaves by using hyper spectral image and high performance liquid chromatography (HPLC) was done. One hundred and twenty hyper-spectral images of cucumber leaves were collected by near infrared hyper-spectral camera, then using HPLC to accurate detect the content of chlorophyll a, chlorophyll b, β-carotene and lutein. After hyper-spectral images have been corrected, a 50×50 pixels region as region of interest (ROI) was defined. Average spectrum from ROI was extracted and prediction model of partial least squares (PLS) with the content of four pigments was built. In order to advance the stability and prediction accuracy, interval partial least squares(iPLS) , backwards interval partial least squares(BiPLS) and synergy interval partial least squares(SiPLS) algorithm was used to select diagnostic bands of each pigments after optimized the number of spectrum interval. The result showed that using BiPLS and SiPLS would get the best PLS model, the best prediction coefficient of chlorophyll a, chlorophyll b, β-carotene and lutein is 0.8257, 0.8134, 0.8116 and 0.8262, respectively.

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鄒小波,陳正偉,石吉勇,黃曉瑋,張德濤.基于近紅外高光譜圖像的黃瓜葉片色素含量快速檢測(cè)[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2012,43(5):152-156. Zou Xiaobo, Chen Zhengwei, Shi Jiyong, Huang Xiaowei, Zhang Detao. Rapid Detection of Cucumber Leaves Pigments Based on Near Infrared Hyper-spectral Image Technology[J]. Transactions of the Chinese Society for Agricultural Machinery,2012,43(5):152-156.

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  • 在線發(fā)布日期: 2012-06-07
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