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基于3,5-二硝基水楊酸法的水稻秸稈酶解工藝
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江蘇省科技支撐計劃資助項目(BE2010356);江蘇省農(nóng)機三項工程資助項目(NJ2010—42)


Enzymatic Hydrolysis of Rice Straw Based on 3,5-dinitrosalicylic Acid Method
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    摘要:

    為了提高汽爆水稻秸稈還原糖的轉(zhuǎn)化率,采用纖維素酶對水稻秸稈進行酶解實驗。還原糖的含量用3,5-二硝基水楊酸法進行測定。實驗選用加酶量、酶解時長和反應(yīng)溫度作為考察因素,以原始水稻秸稈與蒸汽爆破預(yù)處理水稻秸稈作對比。結(jié)果表明纖維素酶用量占秸稈干物質(zhì)質(zhì)量的10%,酶解時長48h,反應(yīng)溫度50℃是一個較為理想的反應(yīng)條件;原始水稻秸稈最大酶解還原糖產(chǎn)量約為9.7%;蒸汽爆破水稻秸稈最大酶解還原糖產(chǎn)量約為34.3%;蒸汽爆破預(yù)處理能夠顯著提高水稻秸稈的酶解還原糖產(chǎn)量,并縮短酶解反應(yīng)時間。

    Abstract:

    For the purpose to improve the conversion rate of reducing sugar of rice straw,the cellulose was used in the enzymatic hydrolysis of rice straw. The content of reducing sugar was detected by 3,5-dinitrosalicylic acid method. The enzyme concentration, hydrolysis time and temperature were selected as inspecting factors. Meantime, comparisons of the enzymatic hydrolysis of rural rice straw with that of steam explosion pretreatment rice straw were made. The results showed when the cellulose weight content taking 10% of the dry rice straw, enzymatic hydrolysis time of 48h and reaction temperature of 50℃ were ideal reaction conditions. The maximum reducing sugar yield of enzymatic hydrolysis original rice straw was 9.7%. The highest reducing sugar yield of enzymatic hydrolysis steam explosion rice straw was approximately 34.3%. Steam explosion pretreatment can obviously improve the production of reducing sugar of enzymatic hydrolysis rice straw, and at the same time accelerate the reaction rate. 

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李彬,高翔,孫倩,陳坤杰.基于3,5-二硝基水楊酸法的水稻秸稈酶解工藝[J].農(nóng)業(yè)機械學報,2013,44(1):106-112. Li Bin, Gao Xiang, Sun Qian, Chen Kunjie. Enzymatic Hydrolysis of Rice Straw Based on 3,5-dinitrosalicylic Acid Method[J]. Transactions of the Chinese Society for Agricultural Machinery,2013,44(1):106-112.

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  • 在線發(fā)布日期: 2012-12-31
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