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Sweet cherry fruit (Prunus avium L. cv. Sunburst) were held into auto-modified atmosphere box (MAB). The postharvest qualities and antioxidative enzymes of sweet cherry fruit were investigated at interval. Results showed that CO2 and O2 concentration in MAB were kept at about 11%~12% and 9%~10% after 30d of storage of sweet cherry fruit. Firmness in fruit stored in MAB was higher than those in control during storage. MAB treatment maintained the higher Vitamin C content, and was effective in inhibition of the declining of soluble solids content in contrast to control, and increased peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX) activity in the early storage. But MAB treatment delayed the increase of pH value, anthocyanin and total phenol content of sweet cherry fruit. MAB treatment inhibited significantly the decay incidence of sweet cherry fruit during storage. The sweet cherry fruit treated by MAB still had good sensory quality significantly after 60d of storage.