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超高壓處理對(duì)S-卵白蛋白構(gòu)象與功能特性的影響
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現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專項(xiàng)資金資助項(xiàng)目(CARS—41—K23)


Effect of Ultra High Pressure on Conformation and Functional Properties of S-ovalbumin
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    摘要:

    采用激光拉曼光譜、熒光光譜分析超高壓(UHP)對(duì)S-卵白蛋白構(gòu)象的影響,并考察其對(duì)溶解性、乳化性和起泡能力等功能特性的影響。結(jié)果表明,經(jīng)UHP處理后S-卵白蛋白的分子量分布沒有明顯變化。隨著壓力增加,無規(guī)卷曲含量逐漸增加,二硫鍵構(gòu)象變化使蛋白穩(wěn)定性降低,酪氨酸殘基包埋于側(cè)鏈中,緊密折疊結(jié)構(gòu)得以舒展。經(jīng)100MPa處理后S-卵白蛋白的熒光強(qiáng)度略有增強(qiáng),此后急劇下降,無峰位位移現(xiàn)象,說明UHP引起蛋白分子構(gòu)象變化。S-卵白蛋白的溶解性、乳化性及起泡能力等功能特性均隨壓力增加而明顯改善,以300MPa處理較為理想,此后壓力增加至500MPa時(shí)反而下降。

    Abstract:

    The effect of ultra high pressure (UHP) treatment on conformation of S-ovalbumin was studied with Raman spectra and fluorescence spectra analysis. The effect of UHP on some functional properties of S-ovalbumin, such as solubility, emulsibility and foamability, were also investigated. It was showed that no significant changed in the molecular weight distribution of S-ovalbumin after UHP. Along with the increase of pressure, the content of random coil of S-ovalbumin was increasing gradually, the stability decreasing owing to the conformation change of disulfide bonds, and tyrosine was buried in the side chain, accordingly the compactly plate sheet structure was to become loose. The fluorescence intensity showed a slight increase after UHP under 100MPa, but then a sharp decline with the increase of pressure, no shift of the fluorescence emission peak happening, so it was pointed out that UHP induced conformational change. The solubility, emulsibility and foamability properties of S-ovalbumin had been improved significantly with the increase of pressure, and achieved the best effect at 300MPa. Then, there was a drop down when the pressure was 500MPa.

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黃群,金永國,馬美湖,張曉維,耿放,孫術(shù)國.超高壓處理對(duì)S-卵白蛋白構(gòu)象與功能特性的影響[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2013,44(3):161-166. Huang Qun, Jin Yongguo, Ma Meihu, Zhang Xiaowei, Geng Fang, Sun Shuguo. Effect of Ultra High Pressure on Conformation and Functional Properties of S-ovalbumin[J]. Transactions of the Chinese Society for Agricultural Machinery,2013,44(3):161-166.

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  • 在線發(fā)布日期: 2013-02-25
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