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玉米粉中黃曲霉毒素B1微波降解技術
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國家自然科學基金資助項目(31071550)、“十二五”國家科技支撐計劃資助項目(2012BAK17B06、2012BAD31B01)和校基本科研青年資助項目(7109021104)


Degradation of Aflatoxin B1 in Corn Flour by Microwave Radiation
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    摘要:

    應用微波輻照技術降解玉米粉中黃曲霉毒素B1(AFB1),采用免疫親和柱凈化-高效液相色譜-柱后衍生的方法測定玉米粉中AFB1的含量。試驗考察了微波輻照功率、微波輻照時間以及AFB1初始含量對其降解效率的影響,并與傳統(tǒng)加熱法進行比較。結果表明:微波輻照主要利用非熱效應對AFB1進行降解,當功率為250 W、時間為30 min時,AFB1降解率大于90%,殘留量在國標和歐盟安全限量范圍之內(nèi)。微波輻照處理對玉米粉營養(yǎng)品質(zhì)影響較小,且AFB1降解產(chǎn)物毒性明顯降低,不會產(chǎn)生二次污染。

    Abstract:

    A microwave irradiation technique was applied for degradation of aflatoxin B1(AFB1) in corn flour, and immunoaffinity column clean-high performance liquid chromatography-post-column derivatization method was applied to determine the content of AFB1. The effects of microwave power, irradiation time and initial quality of AFB1 on degradation efficiency were investigated. Microwave irradiation was compared with conventional heating method. The results showed that the degradation of AFB1 was performed by non-thermal effect. The optimized microwave power was 250 W and irradiation time was 30 min. Under this condition, the degradation rate would reach to 90%. The residues of AFB1 were in the safety limit range of the national standard and the European Union. The nutrition quality of corn flour was slightly affected by the microwave irradiation treatment. The toxicity of AFB1 was reduced obviously and there was no secondary pollution. Therefore, the microwave irradiation can be used as an effective method for the degradation of AFB1. 

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王周利,王玉堂,劉學波,崔璐,劉朝霞,岳田利.玉米粉中黃曲霉毒素B1微波降解技術[J].農(nóng)業(yè)機械學報,2013,44(12):193-197. Wang Zhouli, Wang Yutang, Liu Xuebo, Cui Lu, Liu Zhaoxia, Yue Tianli. Degradation of Aflatoxin B1 in Corn Flour by Microwave Radiation[J]. Transactions of the Chinese Society for Agricultural Machinery,2013,44(12):193-197.

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  • 在線發(fā)布日期: 2013-12-05
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