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不同離心強(qiáng)度損傷對(duì)綠豆芽抗病生理的影響
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浙江省重大科技專項(xiàng)資助項(xiàng)目(2011C12031)和浙江工商大學(xué)校級(jí)創(chuàng)新基金資助項(xiàng)目(1110XJ511105)


Effects of Different Centrifugal Intensity on Disease Resistance of Mung Bean Germinations
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    對(duì)綠豆芽(Vigna radiata)進(jìn)行了可逆損傷(94g)、輕微損傷(376g)、中度損傷(845g)、嚴(yán)重?fù)p傷(1503g)和破損損傷(3381g)的不同離心強(qiáng)度(加速度)的損傷,g為重力加速度,取9.8m/s2,測(cè)定過氧化氫酶(CAT)、過氧化物酶(POD)、超氧化物歧化酶(SOD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性以及超氧陰離子自由基(O-2)的產(chǎn)生速率、過氧化氫(H2O2)質(zhì)量摩爾濃度、水楊酸(SA)質(zhì)量濃度和木質(zhì)素含量變化。包括可逆損傷在內(nèi)的所有損傷,在損傷初期都會(huì)誘導(dǎo)抗病生理??赡鎿p傷的游離態(tài)SA質(zhì)量濃度、H2O2質(zhì)量摩爾濃度和POD活性顯著上升,O-2的產(chǎn)生速率、總酚質(zhì)量濃度、木質(zhì)素含量、SOD活性、CAT活性、PAL活性和PPO活性變化不顯著;輕微損傷、中等損傷、嚴(yán)重?fù)p傷引起更多抗病生理指標(biāo)顯著變化,特別是輕微和中等損傷的POD活性上升最為顯著;破損損傷的游離態(tài)SA質(zhì)量濃度、H2O2、總酚、木質(zhì)素含量上升最大,但是O-2產(chǎn)生速率、CAT、PAL、PPO活性下降也最大。SOD活性對(duì)損傷敏感性較低。

    Abstract:

    The Catalase(CAT), Peroxidase(POD), Superoxide dismutase(SOD), Polyphenol oxidase(PPO)and Phenylalnine ammonialyase(PAL)activity, the Superoxide anion free radical(O-2)generation rate, the Hydrogen peroxide(H2O2), Salicylic acid and Lignin content of mung bean germinations were detected, which were subjected to different centrifugal intensity (acceleration), such as reversible damage(94g), slight damage(376g), moderate damage(845g), serious damage(1503g), and breakage damage(3381g). One gravitational acceleration (g)=9.8m/s2. The results showed that the disease resistance would be induced by all the centrifugal damages including reversible damage in the early stage. The content of free Salicylic acid(SA) and H2O2, POD activity were significantly increased by reversible damage while the change of the O-2 generation rate, total hydroxybenzene, lignin content, the SOD, CAT, PAL and PPO activity were indistinctive. The significant changes of more disease resistance indicators were caused by slight damage, moderate damage and serious damage. Especially the POD activity was increased remarkably under slight damage and moderate damage. The content of free SA, H2O2, total hydroxybenzene and lignin rose in the maximum while the O-2 generation rate, the CAT, PAL and PPO activity declined in the maximum when it was treated with breakage damage. Besides, the SOD activity was not sensitive to all the damages. 

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王向陽,王紅艷.不同離心強(qiáng)度損傷對(duì)綠豆芽抗病生理的影響[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2013,44(12):198-203,214. Wang Xiangyang, Wang Hongyan. Effects of Different Centrifugal Intensity on Disease Resistance of Mung Bean Germinations[J]. Transactions of the Chinese Society for Agricultural Machinery,2013,44(12):198-203,214.

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  • 在線發(fā)布日期: 2013-12-05
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