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In order to investigate the law of ammonia volatilization and nitrogen infiltration in the process of biogas slurry application, the influence rules of different application rate and depth of biogas slurry on the ammonia volatilization of the soil surface and the infiltration of total nitrogen, ammonia nitrogen and nitrate nitrogen in vertical soil profile were explored systematically by soil column experiments at room temperature. The experimental results showed that the total ammonia volatilization of the soil surface and the duration of volatilization were all positively correlated with biogas slurry application rate. The moisture content, total nitrogen and ammonia nitrogen in vertical soil profile were mainly distributed in topsoil. But the nitrate nitrogen had migrated to deep layer soil with the water infiltration of biogas slurry. The total ammonia volatilization of the soil surface decreased with the increase of biogas slurry application depth when the biogas slurry was used as basic fertilizer. The ammonia volatilization rate of biogas slurry could be effectively reduced when the biogas slurry application depth was ten centimeters. And the peaks of total nitrogen, ammonia nitrogen and nitrate nitrogen in vertical soil profile were all positively correlated with biogas slurry application depth.