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The removal of CO2 with aqueous alkanolamine solutions is an important research direction in the field of biogas purification technology, and the research on the kinetics and mass transfer performance of the CO2 absorption is the mainly theoretical basis of optimizing biogas purification process. In the process of using monoethanolamine (MEA) aqueous solution, the Hatta number Ha, enhancement factor E and volumetric overall mass transfer coefficient KGae were studied experimentally at various reaction conditions, such as MEA concentration, gas flow rate, CO2 concentration, and absorption temperature. Furthermore, the impact on the mass transfer performance of reaction and the premise of applying the pseudo first order reaction were also analyzed. The results show that the values of Ha, E and KGae increase with increasing MEA concentration but decrease with increasing CO2 concentration. When the gas flow rate is increased, Ha and E decrease, while KGae initially increase and then decrease. The above parameters increase with increasing absorption temperature, and KGae gradually decrease when the temperature is higher than 57.4℃. Under the experimental conditions, only when the absorbent concentration is higher than 2.5mol/L or the gas flow rate is lower than 5.73kmol/(m2·h), the reaction of MEA and CO2 can be described as a pseudo-first-order reaction. The results can provide a reference for the optimization and development of biogas purification technology.