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人工貴腐葡萄酒香氣的儀器分析與感官評價
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國家自然科學(xué)基金項目(31371724)


Instrumental and Sensory Aroma Analysis of Noble-rot Wine from Artificial Botrytized Grapes
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    摘要:

    研究釀酒葡萄經(jīng)人工侵染灰葡萄孢( Botrytis spp .)后所釀貴腐酒的香氣特征,旨在正確評價該技術(shù)釀造貴腐葡萄酒工藝的可行性。釀酒原料采用陜西楊凌地區(qū)正常采摘的愛格麗葡萄,采用β-葡萄糖苷酶活性較高的優(yōu)選灰葡萄孢菌株人工侵染葡萄和葡萄汁,以甜白葡萄酒工藝釀造葡萄酒,以傳統(tǒng)甜白葡萄酒和風(fēng)干原料甜白葡萄酒為對照。釀造次年對酒樣進(jìn)行GC-MS分析,同時培訓(xùn)品嘗員進(jìn)行香氣特征的感官量化分析。人工貴腐葡萄酒中高級醇含量較低,而酯類和脂肪酸含量較高,乙酸酯類最為突出。人工貴腐葡萄所釀酒和風(fēng)干原料葡萄酒中含有更多的品種香氣成分,尤其是萜烯醇類和去甲類異戊二烯類化合物,因此對葡萄進(jìn)行灰霉菌侵染和風(fēng)干過熟處理均能促進(jìn)香氣前體物質(zhì)的水解。本試驗中,羥苯甲酯僅在人工貴腐葡萄酒中存在,是其典型成分,而人工貴腐葡萄所釀酒中特有的香氣成分還有γ-癸內(nèi)酯、苯乙酸和2-壬酮。感官分析結(jié)果表明,人工貴腐葡萄所釀酒具有明顯的焦糖、甜杏、芒果、烘烤的香氣特征,風(fēng)味特征最為復(fù)雜,而人工貴腐葡萄汁所釀酒香氣特征及其強(qiáng)度相對較弱。所以,經(jīng)灰葡萄孢侵染葡萄所釀貴腐葡萄酒具有典型香氣成分,風(fēng)味復(fù)雜純正,該技術(shù)具有應(yīng)用的可行性。

    Abstract:

    It is known to all that noble-rot wine, a sweet wine made from botrytized grapes, has high cost for its special flavor. Aromas of sweet wines made from artificially botrytized grapes and botrytized juice were studied to evaluate the winemaking process of botrytized wines. Normally harvested Vitis vinifera Var. Ecolly grape was obtained in Yangling, Shaanxi Province, China. The grapes and juice were botrytized artificially by Botrytis spp. with high level of glycosidase activity. The winemaking process normally used for making sweet wine was used to make the artificial noble-rot wines. Normal sweet white wine and wine made from air-dried grapes were used as controls. The aromas of wine samples taken in April of the following year were subjected to chemical and sensory analyses. Botrytis-infected wines contained few higher alcohols but contained high esters and fatty acids, especially acetate esters, which were more likely due to high volatile acids induced by fungal infection. More varietal aroma-active compounds, especially terpenols and norisoprenoids, were detected in wines made from botrytized grapes and air-dried grapes than those in normal sweet white wine and botrytized juice made wine. The treatment of over-ripe grapes promoted hydrolysis of aroma precursors, meanwhile, the metabolic activity of B. cinerea also promoted the hydrolysis. Moreover, hydroxy benzoate esters were typical compounds in botrytis-infected wines and γ-decalactone, phenyl acetic acid and 2-nonanone were unique in wine made from botrytized grapes. The wine made from botrytized grapes had the most complex aroma profile, with sweet fruity, caramel, mango and toast flavors; wine made from botrytized juice had fewer aromas. Therefore, grapes botrytized artificially with B. cinerea could be used to make noble-rot wines with high aroma quality.

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陶永勝,劉吉彬,蘭圓圓,陳超奇,李愛華.人工貴腐葡萄酒香氣的儀器分析與感官評價[J].農(nóng)業(yè)機(jī)械學(xué)報,2016,47(2):270-279,315. Tao Yongsheng, Liu Jibin, Lan Yuanyuan, Chen Chaoqi, Li Aihua. Instrumental and Sensory Aroma Analysis of Noble-rot Wine from Artificial Botrytized Grapes[J]. Transactions of the Chinese Society for Agricultural Machinery,2016,47(2):270-279,315.

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  • 收稿日期:2015-09-21
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  • 在線發(fā)布日期: 2016-02-25
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