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稻秸好氧厭氧兩相發(fā)酵工藝與產(chǎn)氣特性研究
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“十二五”國家科技支撐計劃項目(2014BAD02B04)、東北農(nóng)業(yè)大學博士啟動基金項目(2012RCB72)和哈爾濱市應(yīng)用技術(shù)研究與開發(fā)項目(2013RFQXJ094)


Aerobic and Anaerobic Two-phase Fermentation Process and Biogas Production Characteristic of Rice Straw
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    摘要:

    木質(zhì)纖維素水解困難是制約秸稈沼氣生產(chǎn)效率提高的主要因素,針對秸稈的木質(zhì)纖維素難降解問題,提出濕法好氧厭氧兩相發(fā)酵工藝。在中溫37℃的條件下,水解產(chǎn)酸發(fā)酵容器不密封,采用攪拌供氣、不供氣的好氧水解方式,進行兩相發(fā)酵實驗。結(jié)果表明,好氧厭氧兩相發(fā)酵工藝能夠顯著破壞稻秸的木質(zhì)素結(jié)構(gòu),提高木質(zhì)素降解率,其攪拌供氣組的木質(zhì)素降解率可達4.57%,而單相厭氧發(fā)酵結(jié)束時的木質(zhì)素降解率僅為0.13%,兩相發(fā)酵工藝的TS產(chǎn)氣率可達到411.19mL/g,較單相厭氧發(fā)酵高30%左右。表明好氧厭氧兩相發(fā)酵工藝可有效降解木質(zhì)素并提高產(chǎn)氣率。

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    The main factor of hindering straw fermentation is lignocellulose hydrolysis due to the shielding effect of lignin. The shielding effect of lignin stops the extracellular enzymes of microorganisms getting in touch with cellulose leads to hydrolysis of acid fermentation process slowly. So the key to solving the bottleneck of the anaerobic fermentation process of straw is to damage the lignin structure in the hydrolysis stage and promote the contact of hydrolytic bacteria with the cellulose and hemicellulose. The reproductive capacity of aerobic microorganism is much higher than that of anaerobic microorganism and the initial cleavage of lignin requires the molecular oxygen. So the aerobic and anaerobic twophase fermentation process was put forward in order to improve the degradation rate of lignocellulose. The fermentation process was divided into aerobic hydrolysis fermentation and anaerobic methane fermentation in order to degrade lignocellulose of rice straw which was carried out at 37℃. Hydrolysis of acid fermentation tank was not sealed by stirring gas supply or non gas supply. The results showed that lignin structure of rice straw was damaged by aerobic and anaerobic twophase fermentation and higher lignin degradation rate was obtained. Lignin degradation rate of the stirring gas group was 4.57%, while degradation rate of lignin was only 0.13% at the end of the singlephase anaerobic fermentation. The highest biogas yield of TS was about 411.19mL/g at the stirring gas group 30% higher than the singlephase anaerobic fermentation. Therefore aerobic and anaerobic twophase fermentation process could effectively degrade lignin and increase biogas yield of TS.

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李文哲,丁清華,魏東輝,羅立娜,王慶慶,王金秋.稻秸好氧厭氧兩相發(fā)酵工藝與產(chǎn)氣特性研究[J].農(nóng)業(yè)機械學報,2016,47(3):150-157. Li Wenzhe, Ding Qinghua, Wei Donghui, Luo Li’na, Wang Qingqing, Wang Jinqiu. Aerobic and Anaerobic Two-phase Fermentation Process and Biogas Production Characteristic of Rice Straw[J]. Transactions of the Chinese Society for Agricultural Machinery,2016,47(3):150-157.

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  • 收稿日期:2015-09-14
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  • 在線發(fā)布日期: 2016-03-10
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