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Based on the difference of forchlorfenuron residue and organic component, non-destructive identification method of treated and control kiwifruits was studied using hyperspectral imaging system. Firstly, the representative samples were got by field experiment, and the hyperspectral image was got. The instrument parameters of hyperspectral image were as follows: exposure time 10ms, scanning speed 20mm/s and object distance 20mm/s. Secondly, the spectral regions of 1040~1120nm and 1335~1500nm were selected, totally 116 wave bands were produced, and the average hyperspectral value of each sample was calculated. Finally, all data of hyperspectral image were calculated by Matlab, and the non-destructive identification method of two types of kiwifruit was established. The residue of forchlorfenuron was determined by HPLC, organic component in kiwifruit was analyzed according to the national standard. The results showed that correct identification rate of PCA—LDA and support vector machine (SVM) mathematical models was 94.4%; forchlorfenuron residue level of treated kiwifruit and control kiwifruit were 0.03mg/kg and 0mg/kg, respectively; the content of organic components were different, such as total sugar, total phenols, vitamin C, titratable acid，17 kinds of amino acids and eight trace elements. Therefore, the high spectrum technology was an efficient method for non-destructive identification of PGR (plant growth regulation) kiwifruit.