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In order to solve the human cost, low efficiency, nonmechanization and other issues of picking, a Chrysanthemum morifolium combteeth picking machine was designed. The picking machine was mainly composed of combteeth, two chains with installation holes, four sprockets, a driven shaft, separating teeth, a box, a machine frame, four wheels, a stepping motor and control part. The gap between combteeth was larger than the diameter of bud and less than the diameter of tire chrysanthemum. The flowers were forced to take off by the impact. The test showed that Chrysanthemum morifolium combteeth picking machine can take off the Chrysanthemum morifolium flower reliably. The test was done when the flower moisture content was 82.5%. The single test of combteeth gap selected five levels (7.0mm, 7.5mm, 8.0mm, 8.5mm, 9.0mm). The Chrysanthemum morifolium combteeth picking machine worked well at the gap of 7.5~9.0mm. The drop rate was 0~4.06%, the broken rate was 0~100% and the removal rate was 92.43%~98.11%. The orthogonal tests showed the best combination of combteeth gap, separating teeth gap, feeding depth and rotational speed. Four levels were selected such as 7.5mm, 8.0mm, 8.5mm and 9.0mm for combteeth gap. Separating teeth gap included four levels such as 13mm, 15mm, 17mm and 19mm. Four levels (125mm, 150mm, 175mm, 200mm) were chosen for feeding depth. Rotational speed selected four levels such as 30r/min, 40r/min, 50r/min and 60r/min. The test showed that when the combteeth gap was 8.5mm, the separating teeth was 17mm, the feeding depth was 200mm and the rotational speed was 60r/min, Chrysanthemum morifolium combteeth picking machine would work better. The removal rate was 97.61%, the drop rate was 0.65% and the broken rate was 0.03% at this time.