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液氨預(yù)處理工藝對五節(jié)芒酶解糖化效果的影響
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國家自然科學(xué)基金項目(31500491)和浙江農(nóng)林大學(xué)生物質(zhì)利用研究中心預(yù)研項目(2013SWZ03)


Effects of Liquid Ammonia Treatment on Enzymatic Hydrolysis to Fermentable Sugars of Miscanthus floridulus
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    摘要:

    為提高五節(jié)芒可發(fā)酵單糖的產(chǎn)量,采用液氨對其進(jìn)行預(yù)處理。選用預(yù)處理溫度T、五節(jié)芒含水率M、駐留時間t和液氨用量比k為變量因素,在試驗范圍內(nèi)T(70~170 ℃)、M(20%~200%)、t(5~30min)、k(0.5~5.0)通過對各因素的不同取值,分析上述因素對五節(jié)芒酶解效果的影響。結(jié)果表明:在T=130℃、M=80%、t=10min、k=2.0的條件下預(yù)處理,五節(jié)芒72h的葡聚糖和木聚糖酶解率分別為72.1%和82.5%,即100g干基原材料可獲得可發(fā)酵單糖55.8g,是未經(jīng)預(yù)處理樣品酶解率的3.65倍。因此,液氨預(yù)處理能夠有效促進(jìn)五節(jié)芒的酶解單糖轉(zhuǎn)化率。

    Abstract:

    To effectively improve the enzymatic hydrolysis rate of carbohydrates in lignocelluloses, the liquid ammonia treatment (LAT) was employed as pretreatment for Miscanthus floridulus. The pretreatment temperature T, Miscanthus floridulus moisture M, residence time t and the ratio of ammonia to biomass (dry basis) k were chosen as variable factors. In the experimental ranges of T (70~170 ℃), M (20%~200%), t (5~30min) and k (0.5~5.0), the influences of different variable factors on enzymatic hydrolysis of Miscanthus floridulus were investigated. The chemical composition analysis showed that the chemical constitution of Miscanthus floridulus was close to Saccharum arundinaceum and Miscanthus sinensis, and it could be a prior species of plant energy. The pretreatment methods and effects of these biomasses could be used as reference for each other. The parameter optimization results show that the optimal LAT pretreatment conditions for Miscanthus floridulus were 130℃, 80% moisture content, 10min residence time and 2.0 ammonia to biomass ratio. Enzymatic hydrolysis using cocktail enzymes including cellulase, βglucosidase and xylanase for 72h after pretreatment under optimal conditions, the glucan and xylan hydrolysis rates of LATtreated Miscanthus floridulus achieved 72.1% and 82.5%, which were 3.3fold and 4.3fold than those of untreated biomass respectively. The total fermentable monosaccharides after pretreatment under optimal conditions following enzymatic hydrolysis was 55.8g per 100g of dry raw material. Compared with untreated substrates, it was about 3.65fold in sugar yield for LATtreated substrates. As a result, the LAT process significantly reduced the recalcitrance of Miscanthus floridulus and facilitated biomass conversion to fermentable sugars.

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彭何歡,邵千鈞,Farman Ali Chandio,趙 超,李 彬,馬中青.液氨預(yù)處理工藝對五節(jié)芒酶解糖化效果的影響[J].農(nóng)業(yè)機(jī)械學(xué)報,2016,47(7):196-201. Peng Hehuan, Shao Qianjun, Farman Ali Chandio, Zhao Chao, Li Bin, Ma Zhongqing. Effects of Liquid Ammonia Treatment on Enzymatic Hydrolysis to Fermentable Sugars of Miscanthus floridulus[J]. Transactions of the Chinese Society for Agricultural Machinery,2016,47(7):196-201.

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  • 收稿日期:2016-01-19
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  • 在線發(fā)布日期: 2016-07-10
  • 出版日期: 2016-07-10