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Chlorophyll fluorescence is a useful indicator as it gives important information about the stress and health state of plant. In order to detect plant water stress and growth state accurately and intuitively, plants under different drought stress conditions were experimented by using chlorophyll fluorescence imaging method combined with the chlorophyll fluorescence kinetics parameters. The chlorophyll fluorescence was excited by 460nm LEDs, and acquired by EMCCD equipped with 690nm band pass filter. The average pixel value was calculated for each image of 4000 pictures which were acquired in four different sampling periods individually. The intensity of each image was plotted according to the time sequence, thus the chlorophyll fluorescence kinetics curve could be concluded, and chlorophyll fluorescence kinetics parameters could be calculated. Meanwhile, images were processed for noise reduction, calculation of multiplication and division and so on. And then they were displayed by pseudo color, thus various factors led to uneven distribution of fluorescence could be intuitively analyzed. Fast water stress and slow water stress experiments were conducted for easy to dehydrate and not easy to water loss leaves, respectively. The results showed that the fluorescence ratio Rfd and the time of second peak of kinetics curve were changed with the increase of stress, and both had high fitting determination coefficient with leaf water content (0.86 and 0.98). Therefore, the method combined the chlorophyll fluorescence imaging with chlorophyll fluorescence kinetics parameters could improve the accuracy and intuition of plant drought stress earlier prediction, and it could be a rapid means of remote sensing for site continuous monitoring the growth status of plants, all kinds of stress, pest and disease and so on.