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秸稈濕貯存過(guò)程添加劑協(xié)同調(diào)控對(duì)甲烷產(chǎn)量的影響
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國(guó)家自然科學(xué)基金青年科學(xué)基金項(xiàng)目(51608523)、“十二五”國(guó)家科技支撐計(jì)劃項(xiàng)目(2015BAD21B04)和北京市科技計(jì)劃項(xiàng)目(D161100006016001)


Effect of Wet-storage Additives on Fermentation Performance and Biomethane Potential of Corn Stover
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    摘要:

    以干黃玉米秸稈為原料,研究可溶性糖(葡萄糖)及其與同型乳酸菌、異型乳酸菌、乙酸協(xié)同添加對(duì)原料濕貯存過(guò)程中品質(zhì)及甲烷產(chǎn)量的影響。結(jié)果表明:?jiǎn)我黄咸烟翘砑咏M,貯存30d,干物質(zhì)損失率為4.7%,以乳桿菌屬、片球菌屬、腸球菌多種乳酸菌主導(dǎo),乳酸與乙酸含量(干基質(zhì)量比)分別為36g/kg和5g/kg,秸稈產(chǎn)甲烷潛力比空白組提高68%;葡萄糖植物乳桿菌協(xié)同(同型發(fā)酵)組,貯存30d,干物質(zhì)損失率為4.6%,乳酸含量升高至62.g/kg;〖JP〗葡萄糖短乳桿菌協(xié)同(異型發(fā)酵)組,貯存30d,干物質(zhì)損失率為5.5%,乳酸與乙酸含量分別達(dá)到44g/kg和12g/kg;兩類(lèi)乳酸菌的協(xié)同添加均未對(duì)產(chǎn)氣潛力產(chǎn)生進(jìn)一步促進(jìn)作用;葡萄糖乙酸協(xié)同組,貯存30d,干物質(zhì)損失率為36%,乳酸與乙酸含量分別升高至75g/kg和20g/kg,異型乳酸發(fā)酵魏斯氏菌屬豐度升高至16.87%,有效抑制腸桿菌屬(腐敗菌)生長(zhǎng),秸稈產(chǎn)甲烷潛力提高8.7%。綜合分析本試驗(yàn)結(jié)果,當(dāng)原料可溶性糖含量不足時(shí),外源糖的補(bǔ)充是保證原料高效貯存及產(chǎn)氣的關(guān)鍵,協(xié)同乙酸添加可強(qiáng)化以魏斯氏菌屬為主導(dǎo)的異型發(fā)酵過(guò)程抑制腐敗菌生長(zhǎng),強(qiáng)化貯存過(guò)程的穩(wěn)定性。

    Abstract:

    Corn stover was one of important agricultural residues. Due to Chinese corn farming system, harvesting method, relativity lower mechanism and so on, the corn material for biogas industries was mostly dryyellow corn stover. In order to improve the efficiency of dryyellow corn stover and attain better material for wetstorage, the effect of different wetstorage additives on fermentation performance, bacterial community and biomethane potential (BMP) was focused on. Dryyellow corn stover was stored following five additives treatments for 30d, including Con group (without additives), Glu group (glucose addition of 3%), LP group (glucose addition of 3% and Lactobacillus plantarum addition of 1×106CFU/g), LB group (glucose addition of 3% and Lactobacillus brevis addition of 1×106CFU/g), AA group (glucose addition of 3% and acetic acid addition of 04%). In general, glucose addition 3% prior to storage attained wellpreserve; Lactobacillus plantarum or Lactobacillus brevis addition 1×106CFU/g with glucose addition 3% prior to storage improved the speed of pH value decreasing in early period and water soluble carbohydrate consuming, and significantly higher (P<0.05) lactic acid and acetic acid was produced, respectively. The result of bacterial community from raw material and five treatments showed the most epiphytic bacteria (at the phylum level) was Proteobacteria in raw material, after wetstorage Firmicutes became the dominant flora and Proteobacteria was reduced significantly (P<0.05). The community of lactic acid bacteria showed that Lactobacillus, Enterococcus and Pediococcus were dominant flora when adding glucose alone, and relative abundance of Lactobacillus was increased to 41.07% when adding Lactobacillus plantarum with glucose addition. Weissella became the dominant flora when acetic acid was added. The result of batch test demonstrated that the biomethane potential of dryyellow corn stover was improved by wetstorage with glucose additive. Modified Gompertz models well simulated the anaerobic digestion process of the wetstorage dryyellow corn stover supplemented with additives. Considering the fermentation characteristics, biomethane potential, wetstorage the dryyellow corn stover with glucose addition and acetic acid addition could achieve the well quality and highvalue substance and increase the methane potential yield. 

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崔 憲,郭建斌,徐 艷,溫嘯宇,龐昌樂(lè),董仁杰.秸稈濕貯存過(guò)程添加劑協(xié)同調(diào)控對(duì)甲烷產(chǎn)量的影響[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2018,49(9):302-310. CUI Xian, GUO Jianbin, XU Yan, WEN Xiaoyu, PANG Changle, DONG Renjie. Effect of Wet-storage Additives on Fermentation Performance and Biomethane Potential of Corn Stover[J]. Transactions of the Chinese Society for Agricultural Machinery,2018,49(9):302-310.

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  • 收稿日期:2018-02-02
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  • 在線(xiàn)發(fā)布日期: 2018-09-10
  • 出版日期: 2018-09-10
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