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魚油納米乳液運(yùn)載體系構(gòu)建與穩(wěn)定性研究
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國家自然科學(xué)基金面上項(xiàng)目(31571876、31671807)


Formation and Stability of Fish Oil Enriched Biocompatible Nano-emulsion
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    摘要:

    以大豆蛋白-磷脂酰膽堿作為復(fù)合乳化劑,采用高壓均質(zhì)技術(shù)制備魚油納米乳液,研究了大豆蛋白質(zhì)量分?jǐn)?shù)、磷脂酰膽堿質(zhì)量分?jǐn)?shù)、魚油質(zhì)量分?jǐn)?shù)、均質(zhì)壓力對(duì)魚油納米乳液平均粒徑、PDI、ζ-電位、濁度等性質(zhì)影響,確定了最佳制備工藝參數(shù)為:大豆蛋白質(zhì)量分?jǐn)?shù)2%,磷脂酰膽堿質(zhì)量分?jǐn)?shù)0.2%,魚油質(zhì)量分?jǐn)?shù)1.5%,均質(zhì)壓力100MPa,得到魚油納米乳液的平均粒徑為(245±3.1)nm,PDI為0.226±0.019,ζ-電位為(-30.2±0.6)mV,濁度為(2413±34.7)cm-1。通過超高分辨顯微鏡觀測(cè)到魚油被包埋于復(fù)合乳化劑中且均勻分布在乳液體系中;通過穩(wěn)定性研究發(fā)現(xiàn):大豆蛋白-磷脂酰膽堿魚油納米乳液分別在4℃和25℃儲(chǔ)存30d均穩(wěn)定;對(duì)一定濃度的Na+有較好的抗性;酸性條件不穩(wěn)定,堿性條件下穩(wěn)定。

    Abstract:

    Soybean protein-phosphatidylcholine was used as the emulsifier to prepare fish oil nano-emulsion by high pressure homogenization technology. The effect of concentrations of soybean protein, phosphatidylcholine and fish oil, and homogenous pressure on the average particle size, PDI, ζ-potential, turbidity of fish oil nano-emulsion were studied. Results with the highest desirability (the mean droplet size was (245±3.1)nm, PDI was 0.226±0.019, ζ-potential was (-30.2±0.6)mV and turbidity was (2.413±34.7)cm-1 was obtained with mass ratio of soybean protein content of 2%,phosphatidylcholine content of 0.2%, fish oil content of 1.5%, and homogenization pressure of 100MPa.Through the super high-resolution microscopy it was observed that fish oil was embedded in the composite emulsifier and evenly distributed in the emulsion system. Through the stability study of the highest desirability soybean protein-phosphatidylcholine fish oil nano-emulsion, it was found that nano-emulsion was remained stable against coalescence and phase separation for a period of 30d (storage period) at 4℃ and 25℃, respectively. The ability of the soybean protein-phosphatidylcholine fish oil nano-emulsion was stronger than that of Tween20 fish oil nano-emulsion. The highest desirability soybean protein-phosphatidylcholine fish oil nano-emulsion had good ionic resistance ability, and the influence of Ca2+ on stability of nano-emulsions was greater than Na+. In addition, the nano-emulsion was stable under all processing conditions that encountered during food processing at pH value of 7.0~10.0. The developed fish oil nano-emulsion had potential applications in the food industry.

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江連洲,綦玉曼,馬春芳,劉寶華,王中江,李楊.魚油納米乳液運(yùn)載體系構(gòu)建與穩(wěn)定性研究[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2018,49(10):387-395. JIANG Lianzhou, QI Yuman, MA Chunfang, LIU Baohua, WANG Zhongjiang, LI Yang. Formation and Stability of Fish Oil Enriched Biocompatible Nano-emulsion[J]. Transactions of the Chinese Society for Agricultural Machinery,2018,49(10):387-395.

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  • 收稿日期:2018-04-28
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  • 在線發(fā)布日期: 2018-10-10
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