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解淀粉芽孢桿菌L-S60與黃瓜互作特性研究
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國(guó)家自然科學(xué)基金項(xiàng)目(31671831)和公益性行業(yè)(農(nóng)業(yè))科研專(zhuān)項(xiàng)(201303014)


Characteristics of Interaction between Bacillus amyloliquefaciens L-S60 and Cucumbers
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    摘要:

    植物根系分泌物作為植物與土壤進(jìn)行物質(zhì)交換和信息傳遞的重要載體物質(zhì),對(duì)植物-微生物種間互作發(fā)揮著重要作用。本文分析了解淀粉芽孢桿菌L-S60與黃瓜的互作過(guò)程,研究了黃瓜根系分泌物的主要成分有機(jī)酸及氨基酸對(duì)菌株的趨化作用及菌株的利用情況,通過(guò)計(jì)數(shù)和熒光定量PCR的方式分析了菌株在黃瓜幼苗的根際定殖情況。結(jié)果表明,L-S60能夠部分利用黃瓜根系分泌物中主要的有機(jī)酸(檸檬酸、草酸、琥珀酸和蘋(píng)果酸)及氨基酸(谷氨酸、苯丙氨酸、半胱氨酸和色氨酸)成分并且受到這些成分的趨化作用,其中對(duì)于蘋(píng)果酸和谷氨酸的利用情況及受趨化作用最優(yōu)。從定殖量及相關(guān)基因轉(zhuǎn)錄水平差異上分析,菌株具有在黃瓜幼苗根系表面定殖的能力,且菌株最大定殖量為1.02×105CFU/g。

    Abstract:

    Plant root exudate plays an important role in the process of material exchange between plant and soil. Moreover, it is a significant factor in ‘plantmicroorganisms’ interactions. The process of interaction between Bacillus amyloliquefaciensL-S60 and cucumbers was analyzed. The utilization and chemotaxis of the main components of cucumber root exudate, organic acids and amino acids, by L-S60 were studied. After establishing the interaction model between the strain and the cucumber seedlings, the coloning of root surface in hydroponic culture was studied via counting the strain by time. Furthermore, the genes related to root colonization, swarming motility and biofilm formation on translational level were analyzed by fluorescent quantitation PCR. The results showed that L-S60 could utilize the main organic acids (citric acid, oxalic acid, succinic acid and malic acid) and amino acids (glutamic acid, phenylalanine, cysteine and tryptophan) from cucumber root exudates and provide well utilization of malic acid and glutamic acid. Also, L-S60 showed the chemotaxis toward these components and could be strongly attracted to malic acid and glutamic acid. According to the CFUs, which could colonize on the root surface, and the relative quantitative expression of the related genes on translational level, L-S60showed strong colonization capacities on the cucumber root surface and the most amount of colonization was 1.02×105CFU/g. In conclusion, L-S60 showed great potential to become a biological control agent of practical applications in the future. 

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張瑩,秦宇軒,尚慶茂,張一凡,李昌輝,李平蘭.解淀粉芽孢桿菌L-S60與黃瓜互作特性研究[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2019,50(2):258-265. ZHANG Ying, QIN Yuxuan, SHANG Qingmao, ZHANG Yifan, LI Changhui, LI Pinglan. Characteristics of Interaction between Bacillus amyloliquefaciens L-S60 and Cucumbers[J]. Transactions of the Chinese Society for Agricultural Machinery,2019,50(2):258-265.

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  • 收稿日期:2018-08-11
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  • 在線發(fā)布日期: 2019-02-10
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