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大豆肽-鈣螯合物制備條件優(yōu)化與質(zhì)量評(píng)價(jià)
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國(guó)家重點(diǎn)研發(fā)計(jì)劃項(xiàng)目(2016YFD0400605)和黑龍江省自然科學(xué)基金項(xiàng)目(QC2016039)


Optimization of Parameters of Preparation of Calciumchelated Soybean Peptides and Quality Evaluation
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    摘要:

    采用堿性蛋白酶對(duì)大豆脫脂豆粕進(jìn)行酶解,探究大豆肽-鈣螯合物的最佳制備工藝參數(shù),并對(duì)大豆肽-鈣螯合物的性質(zhì)進(jìn)行分析。以鈣結(jié)合量作為指標(biāo),研究肽鈣比、反應(yīng)溫度、反應(yīng)時(shí)間和pH值對(duì)鈣結(jié)合量的影響,并通過(guò)Box-Benhnken中心組合試驗(yàn)以及響應(yīng)面分析法對(duì)螯合工藝進(jìn)行優(yōu)化。結(jié)果表明,最佳螯合工藝為:肽鈣比1.6、反應(yīng)溫度38℃、反應(yīng)時(shí)間44min、pH值6.6,在此條件下制得的大豆肽-鈣螯合物的鈣結(jié)合量為78.73mg/g。經(jīng)試驗(yàn),測(cè)得大豆肽-鈣螯合物的溶解度為98.59%,分子量大于5000Da的大豆肽-鈣合物占1.76%,分子量小于5000Da的占98.24%,分子量小于1000Da的大豆肽-鈣螯合物占80.24%,其中分子量180~500Da的大豆肽-鈣螯合物占比最大。本研究可為新型大豆?fàn)I養(yǎng)保健產(chǎn)品及合成新型補(bǔ)鈣制劑的研發(fā)提供新方法、新思路。

    Abstract:

    The defatted soybean meal was hydrolysised by alcalase to explore the optimal preparation parameters of calciumchelated soybean peptides, and the properties of alciumchelated soybean peptides were analyzed. The effects of mass ratio of peptide and calcium, chelating temperature, pH and chelating time on calcium binding capacity were studied, and the chelating process was optimized by Box-Benhnken experimental design and response surface methodology (RSM). The results showed that the optimum chelating conditions were as follows: ratio of peptide to calcium was 16, chelating temperature was 38℃, chelating time was 44min, and pH value was 66. Under the optimal conditions, the calcium content was the highest (7873mg/g) in calciumchelated soybean peptides. Moreover, it was concluded that the solubility of the finally obtained calciumchelated soybean peptides was 9859%. Furthermore, the molecular mass distribution of soybean peptidecalcium chelate was also determined by highperformance liquid chromatography (HPLC). The proportion of peptides with a relative molecular weight higher than 5000Da was 176%, peptides with a relative molecular weight lower than 5000Da accounted for 9824%, and peptides with molecular weight lower than 1000Da accounted for 8024%. Among them, the peptides with the relative molecular weight of 180~500Da accounted for the largest proportion. It provided a new method for the research and development of new soybean nutrition and synthesis of new calcium supplements.

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李國(guó)良,侯俊財(cái).大豆肽-鈣螯合物制備條件優(yōu)化與質(zhì)量評(píng)價(jià)[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2019,50(8):379-385,409. LI Guoliang, HOU Juncai. Optimization of Parameters of Preparation of Calciumchelated Soybean Peptides and Quality Evaluation[J]. Transactions of the Chinese Society for Agricultural Machinery,2019,50(8):379-385,409.

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  • 收稿日期:2019-05-13
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  • 在線發(fā)布日期: 2019-08-10
  • 出版日期: 2019-08-10
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