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益生菌發(fā)酵蘋果汁過程中總酚酸變化與動力學研究
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國家重點研發(fā)計劃項目(2017YFD0400700、2017YFD0400702)


Changes and Kinetics of Total Phenolic Acids of Apple Juice Fermented by Probiotics
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    摘要:

    為了使蘋果汁中結(jié)合態(tài)多酚變?yōu)橛坞x態(tài)多酚,提高蘋果汁的功能性多酚單體含量,利用嗜酸乳桿菌6005、植物乳桿菌21805和發(fā)酵乳桿菌21828混菌發(fā)酵復合蘋果汁,分析蘋果汁發(fā)酵過程中活菌數(shù)及理化成分變化,建立混菌生長和總酚酸變化動力學模型,并進行模型驗證。結(jié)果表明:混合益生菌在復合蘋果汁中生長良好,活菌數(shù)達到2.68×108CFU/mL,發(fā)酵過程中總糖含量下降,可滴定酸含量上升,總酚酸含量總體呈上升趨勢,綠原酸及沒食子酸等功能性酚酸類多酚單體含量增加;建立了復合益生菌發(fā)酵蘋果汁的菌體生長動力學模型和總酚酸增加量變化動力學模型,模型理論值與試驗值的平均誤差小于10%,說明建立的動力學模型能夠較好地預測混合益生菌發(fā)酵蘋果汁中總酚酸的變化過程。

    Abstract:

    In order to change the binding polyphenols into free polyphenols in apple juice, improve the content of functional polyphenols, and develop products of apple juice with health care function, Lactobacillus acidophilus 6005, Lactobacillus plants 21805 and Lactobacillus fermentum 21828 were mixed and fermented with apple juice, which was mixed with three varieties of Aksu, Qinguan and Granny Smith. The changes of viable cell numbers and physicochemical composition in apple juice fermentation process were studied. In addition, the kinetic model of bacterial growth and the kinetic model of total phenolic acid incremental change were established and verified. The results showed that probiotics grew well in apple juice, and the number of viable bacteria exceeded 2.68×108CFU/mL. During fermentation, the total sugar content was decreased, the titratable acid content was increased, and the total phenolic acid content was increased. Moreover, the content of several functional phenolic polyphenol was increased, such as chlorogenic acid and gallic acid. Probiotic fermentation changed the quality of apple juice. The kinetic model of bacterial growth and the kinetic model of total phenolic acid change were established. The average error between the theoretical and experimental values of the model was less than 10%. The fitting was good and the established kinetic model could predict the fermentation process. The research results provided a theoretical basis for the development of functional juice beverage products.

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高振鵬,宋楊,張美娜,韓夢珍,岳田利.益生菌發(fā)酵蘋果汁過程中總酚酸變化與動力學研究[J].農(nóng)業(yè)機械學報,2019,50(11):350-356. GAO Zhenpeng, SONG Yang, ZHANG Meina, HAN Mengzhen, YUE Tianli. Changes and Kinetics of Total Phenolic Acids of Apple Juice Fermented by Probiotics[J]. Transactions of the Chinese Society for Agricultural Machinery,2019,50(11):350-356.

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  • 收稿日期:2019-09-18
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  • 在線發(fā)布日期: 2019-11-10
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