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基于ATR的玉米種子活力太赫茲特征波段篩選方法
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國(guó)家自然科學(xué)基金項(xiàng)目(61807001)、國(guó)家留學(xué)基金委訪學(xué)項(xiàng)目(2019)和北京工商大學(xué)基本科研業(yè)務(wù)費(fèi)項(xiàng)目(PXM2019_014213_〖JP〗000007)


Screening Method of Characteristic THz Region to Corn Seed Vigor Based on ATR
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    應(yīng)用太赫茲時(shí)域光譜技術(shù)結(jié)合移動(dòng)窗口相關(guān)系數(shù)法篩選與種子老化密切相關(guān)的太赫茲特征波段。選取3個(gè)不同品種的玉米種子,并制備人工老化0、1、2、3、4d的實(shí)驗(yàn)樣本,采用衰減全反射(Attenuated total reflection, ATR)附件采集從樣本分離得到的種胚和胚乳粉末的太赫茲吸光度譜(0.2~80cm-1),應(yīng)用移動(dòng)窗口相關(guān)系數(shù)法(窗口寬度為10,閾值為0.3)篩選樣本種胚和胚乳特征譜區(qū)。結(jié)果表明,不同品種樣本篩選得到的老化特征譜區(qū)差異顯著,說(shuō)明種子老化進(jìn)程受品種影響較大;在相同的老化階段,不同品種樣本的特征譜區(qū)也存在部分共同區(qū)間,且主要集中在60~80cm-1,說(shuō)明老化進(jìn)程中有些成分的變化相近;種胚和胚乳在相同老化階段篩選的特征譜區(qū)不同,說(shuō)明兩者在老化進(jìn)程中生理變化存在差異。本研究表明太赫茲時(shí)域光譜結(jié)合化學(xué)計(jì)量學(xué)方法可用于快速表征并探測(cè)玉米種子老化的動(dòng)態(tài)變化過(guò)程。

    Abstract:

    Characteristic Terahertz (THz) region closely related to corn seed aging were screened by using THz time domain spectroscopy technique and moving window correlation coefficient method. There were three different varieties of corn seeds selected and processed by artificial aging for 0d, 1d, 2d, 3d and 4d. The embryos and endosperms of corn seeds samples were isolated and pulverized. THz absorbance spectra (0.2~80cm-1) of the powder samples were collected by THz time domain system and attenuated total reflection (ATR) accessory. The moving window correlation coefficient method was applied to screen the characteristic spectral regions which were sensitive to seed aging. After comparing the parameters of the moving window correlation coefficient, the window width was determined to 10 and threshold value was 0.3. According to the result, the selected characteristic spectral regions showed significant differences among different varieties of samples, which indicated that the seed aging process was greatly affected by the variety. However, there were minor common regions in the same aging stage which were mainly concentrated in 60~80cm-1. It was showed that the similar components were changed at the same time. Due to the selected characteristic spectra of endosperm and embryo at the same aging stage were different, it was showed that the endosperm and embryo had different physiological changes during seed aging process. The experiment result indicated that THz time domain spectroscopy combined with chemometric method can be used to characterize and detect the dynamic changes of corn seed aging rapidly and macroscopically. 

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吳靜珠,李曉琪,劉翠玲,孫曉榮,余樂(lè),孫麗娟.基于ATR的玉米種子活力太赫茲特征波段篩選方法[J].農(nóng)業(yè)機(jī)械學(xué)報(bào),2020,51(4):382-386,393. WU Jingzhu, LI Xiaoqi, LIU Cuiling, SUN Xiaorong, YU Le, SUN Lijuan. Screening Method of Characteristic THz Region to Corn Seed Vigor Based on ATR[J]. Transactions of the Chinese Society for Agricultural Machinery,2020,51(4):382-386,393.

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  • 收稿日期:2019-11-11
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  • 在線發(fā)布日期: 2020-04-10
  • 出版日期: 2020-04-10
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