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The spatial scale of landscape pattern has become the basis of landscape ecology research. By analyzing the characteristics and changing rules of the same landscape pattern in different scales and clarifying the scale effect of regional landscape pattern characteristics, it can be known more about the real situation of landscape pattern. Wengniute Banner of Chifeng City, Inner Mongolia was taken as the research area. The remote sensing images of the same month in 2008, 2013 and 2018 were used to combine the land use data of Wengniute Banner area. Landscape types were divided into six categories: cultivated land, woodland, grassland, water area, construction land and unused land. Landscape pattern index was analyzed. Six landscape indices at patch type level and six landscape indices at landscape level were selected to analyze the effects of different grain sizes on landscape pattern indices. The results showed that in landscape level pattern index, the number of patches was decreased from 5391 to 4231, the density of patches was decreased from 0.40 per hectare to 0.32 per hectare, the diversity of Shannon was increased from 1.37 to 1.45, and the evenness of Shannon was increased from 0.76 to 0.81, which indicated that the dominant landscape had not yet been formed, and the fragmentation of landscape was increased. At the level of patch type, the percentage of patch area had no obvious change with the change of grain size, and other landscape types were changed with the change of grain size. Among them, in 2013 and 2018, the granularity effect of water area on patch density was strong, which was from 0.005 per hectare to 0.046 per hectare and 0.010 per hectare to 0.046 per hectare, respectively. The granularity effect of unused land on effective network size was strong, which was from 24596m2 to 40697m2. The granularity effect of construction land on aggregation and cohesion was strong, which was from 0.9 to 0.3 and 94% to 49%, respectively.