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The current seedling picking device used for vegetable transplanter was restricted by the working principle and mechanical structure, the frequency of picking seedling was mostly 40~90 plants/min per row, seedling picking had become a bottleneck restricting the development of technology and equipment for high-speed vegetable transplanting (≥90 plants/min). In order to realize the high-speed seedling picking operation, a pneumatic punching high-speed seedling picking device and supporting combined plug tray were designed. Through the operation process of “supplying tray orderly, conveying tray continuously, punching seedlings pneumatically and feeding seedlings freely”, the high-speed seedling picking operation at the frequency of 120 plants/min was realized. The mechanics model of seedlings in the process of punching was constructed, the layout of the pneumatic punching seedling monomer, the working pressure of the seedling picking cylinder, and the movement trajectory of the seedling ejector were analyzed and calculated. The structure and size of the U-shaped end of the ejector were optimized, the high-speed seedling picking sequence control system was designed and built. Taking 60d seedling-age pepper seedlings as the experimental object, under the conditions of cylinder working pressure of 0.26MPa and frequency of picking seedlings at 120 plants/min, seedling picking experiments were carried out by taking seedling picking success rate, matrix damage rate and stem and leaf damage rate as evaluation indicators of seedling picking effect. The result of experiments showed that the average success rate of seedling picking was 100%, the average substrate breaking rate was 22.46%, and the average stem and leaf damage rate was 3.54%, which met the requirements of high-speed seedling picking operation.