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Aiming at the problems of low mechanization degree, low harvest efficiency and high harvest damage rate of Panax notoginseng in greenhouse, a self-propelled greenhouse Panax notoginseng harvest machine with hydraulic power transmission system and 380V three-phase power source was designed, which can complete the harvest of Panax notoginseng, root soil separation and other tasks, its key components were studied. Based on agronomic requirements, the whole machine structure and crawler chassis were designed, and the kinematics model was established. The linear motion characteristics of the complete machine were studied using RecurDyn. The single-side traction power was about 1357N, and the center of mass fluctuation was stable. By establishing the kinematic model of the root-soil mixture, the optimal structural parameters of the excavation were determined. The hydraulic control design was divided into three parts: walking, driving, and lifting. FluidSIM and GX Developer were used to design the local operating system and remote-control system software, respectively. The field test results showed that the hydraulic control system and the mechanical part worked well under the conditions of excavation. Under different control modes, the harvest machine can complete the functions of straight line, turning, lifting, and lifting chain rotation, etc. The straight-line travel was stable, the travel offset was 0.49%, and the forward and backward velocities of the hydraulic cylinder were 0.22m/s, respectively, which were basically consistent with the simulation results. The average yield loss rate was 1.48%, and the average root injury rate was 1.77%. There was no obvious root embedding phenomenon, which met the evaluation index of Panax notoginseng harvest machine.