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The effect of vitamin C on the oscillation of (NH4)4Ce(SO4)4-NaBrO3-malonic acid chemical oscillation system was studied. The blank samples of milk powder and pure water were taken as test samples, and the data were collected by clicking the menu. When the oscillation curve was smooth for 8min, different amounts of vitamin C were added to investigate the influence of each amount of vitamin C on the visual information and characteristic parameters of the corresponding oscillation spectrum. The results showed that when the amount of vitamin C was more than 0.0015g per gram milk powder, the visual spectrum and some characteristic parameters of milk powder and pure water blank samples were changed significantly, especially when the amount of vitamin C was more than 0.0106g, the maximum amplitude of characteristic parameters of milk powder oscillation spectrum after interference was basically unchanged, and the oscillation state basically stopped. For the interference of vitamin C to the blank sample, when the vitamin C content was greater than 0.0203g, the maximum amplitude after interference basically reached a stable state, and the peak valley potential after interference was decreased with the gradual increase of vitamin C content. When the vitamin C content was greater than or equal to 0.0254g, the peak valley potential after interference basically did not change. The research result provided a theoretical basis for the qualitative and quantitative analysis of vitamin C content in milk powder samples.