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To achieve the mechanized integrated transplanting requirement of rice pot seedlings on film, a non-circular gear-linkage combined planetary gear system transplanting mechanism was proposed. This mechanism enabled a single set of components to achieve the coordinated operation of four processes: seedling picking, seedling delivery, film perforation and digging, and planting meeting the trajectory and attitude requirements of rice pot seedling transplanting on film. The transplanting mechanism was theoretically analyzed, and a kinematic model was established. Combining optimization objectives, a digital visualization optimization design software for the mechanism was developed based on the Matlab GUI platform, resulting in a set of mechanism parameters that met the transplanting requirements through human-computer interaction. Based on the optimized parameters, a comprehensive structural design of the mechanism was conducted, and a 3D model was established. Virtual prototype simulation was performed by using ADAMS software. A physical prototype of the rice pot seedling transplanting mechanism on film and a multifunctional test bench were developed. The kinematic characteristics and performance of the transplanting mechanism under idle and seedling-carrying states were studied. The results showed that the theoretical trajectory, virtual prototype simulation trajectory, and physical prototype test bench trajectory remained consistent within an acceptable error range, validating the consistency and correctness of the design of the transplanting mechanism. The performance test showed a seedling extraction success rate of 94%, a transplanting success rate of 92.36%, and a coefficient of variation of planting spacing of 2.67%, meeting the requirements of transplanting operation. This validated the rationality and feasibility of the design of the rice pot seedling transplanting mechanism on film.